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Title of Journal: Cytotechnology

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Abbravation: Cytotechnology

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Springer Netherlands

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DOI

10.1007/bf01097271

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1573-0778

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Optimization of the isolation and expansion method

Authors: Camilla Siciliano Mohsen Ibrahim Gaia Scafetta Chiara Napoletano Giorgio Mangino Luca Pierelli Giacomo Frati Elena De Falco
Publish Date: 2013/12/04
Volume: 67, Issue: 1, Pages: 165-174
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Abstract

Mesenchymal stem cells MSCs are adult multipotent cells currently employed in several clinical trials due to their immunomodulating angiogenic and repairing features The adipose tissue is certainly considered an eligible source of MSCs Recently putative adipose tissue derived MSCs ADMSCs have been isolated from the mediastinal depots However very little is known about the properties the function and the potential of human mediastinal ADMSCs hmADMSCs However the lack of standardized methodologies to culture ADMSCs prevents comparison across Herein for the first time we report a detailed step by step description to optimize the isolation and the expansion methodology of hmADMSCs using a virally inactivated good manufacturing practice GMPgrade platelet lysate highlighting the critical aspects of the procedure and providing useful troubleshooting suggestions Our approach offers a reproducible system which could provide standardization across laboratories Moreover our system is time and cost effective and it can provide a reproducible source of adipose stem cells to enable future studies to unravel new insights regard this promising stem cell populationWe dedicate this manuscript to Maria Pia Docimo We also acknowledge Fondazione Roma We thank Colin Murdoch Rosa Puca and Isotta Chimenti for their help and support This study was supported by the grant University of Rome “Sapienza” Ateneo 2011 prot C26A11J528

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