Authors: Una Riekstina Ruta Muceniece Inese Cakstina Indrikis Muiznieks Janis Ancans
Publish Date: 2009/02/14
Volume: 58, Issue: 3, Pages: 153-162
Abstract
This study investigated conditions for optimal in vitro propagation of human skinderived mesenchymal stem cells SMSC Forty primary skinderived precursor cell SKP cultures were established from both male and female donors age 29–65 years and eight of them were randomly selected for indepth characterization Effects of basic fibroblast growth factor FGF2 epidermal growth factor EGF leukemia inhibiting factor LIF and dibutyrylcyclic adenosine monophosphate dbcAMP on SMSC proliferation were investigated Primary SKP cultures were 95 homogenous for CD90 CD73 and CD105 marker expression enabling to classify these cells as SMSC FGF2 dosedependent stimulation was observed in low serum medium only whereas EGF neither stimulated SMSC proliferation nor potentates the effect of FGF2 Pronounced donor to donor differences among SMSC cultures were observed in 3day proliferation assay This study demonstrates that homogenous SMSC populations can be reproducibly isolated from individual donors of different age Optimal cell culture conditions for in vitro propagation of SMSC are B27 supplemented or low serum media with FGF2 4 ng/ml EGF and LIF as well as dbcAMP are dispensable for SMSC proliferationWe thank Dr Stengrevics Riga Eastern Hospital and Dr Jankovskis Experimental and Clinical Medicine Institute University of Latvia for help with tissue samples and Dr Dambrova and Dr Liepinsh from Latvian Institute of Organic Synthesis for collaboration The presented work was supported by the European Regional Development Fund ERDF project NoVPD/ERAF/CFLA/05/APK/252/000072/036
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