Authors: Vahid Molla Kazemiha Shahram Azari Amir Amanzadeh Shahin Bonakdar Morteza Shojaei Moghadam Mahdi Habibi Anbouhi Susan Maleki Nahid Ahmadi Tahmineh Mousavi Mohammad Ali Shokrgozar
Publish Date: 2011/08/25
Volume: 63, Issue: 6, Pages: 609-620
Abstract
Mycoplasma contamination is a deleterious event for cell culture laboratories Plasmocin™ is used to prevent and eradicate mycoplasma infections from cell In this study 80 different mammalian cell lines from various sources human monkey mice hamster and rat were used to study and evaluate plasmocin™ efficiency and compare it to commonly used antibiotics such as BMcyclin ciprofloxacin and mycoplasma removal agent MRA It was shown that mycoplasma infections were eradicated by plasmocin™ BMcyclin ciprofloxacin and MRA in 65 6625 20 and 3125 respectively of infected cell cultures However reinfection with mycoplasmas after the period of 4 months occurred in 10–80 of the studied cell lines Cell cytotoxicity and culture death was observed in 25 175 and 10 of the treated cells for plasmocin™ BMcyclin and MRA respectively In this study Plasmocin™ showed strong ability to eradicate mollicutes from our cell lines with minimal percentage of regrowth However due to its high cell cytotoxicity it should be used with caution especially when dealing with expensive or hardtoobtain cell lines Amongst the antibiotics tested BMcyclin was shown to remove mycoplasma with the highest efficiencyThe authors would like to express their appreciation to Prof Ehsan Mostafavi head of department of epidemiology and Ali Jahanian Najafabadi for their technical assistance The authors also would like to thank Pasteur Institute for their financial assistance
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