Authors: Xianxiang Xu Xinghua Gao Rong Pan Dan Lu Yue Dai
Publish Date: 2010/03/09
Volume: 62, Issue: 1, Pages: 17-22
Abstract
Cell adhesion plays a key role during various physiological and pathological processes Many studies have been performed to understand the interaction of platelets with endothelial cells ECs during the past decades Modulation of their interaction has been shown to be therapeutically useful in thrombotic diseases Some methods of labeling platelets such as counting and radiolabeling have been applied in the study of the plateletsECs interaction but these methods did not obtain full approval A rapid simple and sensitive assay for plateletsECs interaction was developed in this paper Platelets were labeled with Sudan Black B SBB before adding to confluent ECs monolayer Nonadherent platelets were removed by washing with PBS The adherent platelets were lysed with dimethylsulfoxide DMSO and the absorbance was recorded at 595 nm by spectrophotometer A linear correlation was observed between the absorbance of SBB and the number of platelets By employing the SBB method the influence of heparin on plateletsECs interactions was observed Heparin 3–100 units/mL obviously reduced platelets adhering to ECs in a concentrationdependent manner
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