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Title of Journal: Calcif Tissue Int

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Abbravation: Calcified Tissue International

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Springer-Verlag

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DOI

10.1007/s11095-004-9008-0

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1432-0827

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Optimal LowEnergy Laser Irradiation Causes Tempor

Authors: E Fukuhara T Goto T Matayoshi S Kobayashi T Takahashi
Publish Date: 2006/12/08
Volume: 79, Issue: 6, Pages: 443-450
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Abstract

Lowenergy laser irradiation LELI accelerates wound healing and is thought to accelerate bone formation However the mechanism of laser healing is not clear To clarify the biological mechanism of LELI healing we investigated the effects of LELI on rat osteoblasts in vitro Osteoblastic cells from 3dayold Wistar rat calvaria were irradiated using a lowenergy galliumaluminumarsenide GaAlAs diode laser Bone formation osteoblast differentiation and cell proliferation were evaluated by von Kossa staining reversetranscription polymerase chain reaction alkaline phosphatase ALP staining 5bromo2′deoxyuridine BrdU uptake and fluorescenceactivated cell sorter FACS analysis At 21 days after LELI the greatest bone formation was observed with irradiation energy of 375 J/cm2 and the first week after seeding LELI 375 J/cm2 induced an increased number of cells at day 3 LELIstimulated differentiation in osteoblastic cells was demonstrated by the increases of Runx2 expression and ALPpositive colonies By contrast at 1 day after laser irradiation the number of cells in the irradiation group was significantly lower than that in the control group BrdU uptake indicated lower proliferation 12 and 24 hours after irradiation compared with the control Furthermore FACS data demonstrated a higher proportion of cells in the G2/M phase of the cell cycle 12 hours after irradiation compared with the control G2/M arrest was confirmed by the appearance of G2/M arrest marker 1433σ or phosphop53 These results demonstrate that LELI induces not only acceleration of bone formation but also initial G2/M arrest which may cause wound healing like tissue repair

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