Fourier Transform Infrared Imaging Microspectrosco

Authors: HaydenWilliam Courtland Philip Nasser Andrew B Goldstone Lyudmila Spevak Adele L Boskey Karl J Jepsen

Publish Date: 2008/10/15

Volume: 83, Issue: 5, Pages: 342-

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Abstract

Fracture susceptibility is heritable and dependent upon bone morphology and quality However studies of bone quality are typically overshadowed by emphasis on bone geometry and bone mineral density Given that differences in mineral and matrix composition exist in a variety of species we hypothesized that genetic variation in bone quality and tissuelevel mechanical properties would also exist within species Sixteenweekold female A/J C57BL/6J B6 and C3H/HeJ C3H inbred mouse femora were analyzed using Fourier transform infrared imaging and tissuelevel mechanical testing for variation in mineral composition mineral maturity collagen crosslink ratio and tissuelevel mechanical properties A/J femora had an increased mineraltomatrix ratio compared to B6 The C3H mineraltomatrix ratio was intermediate of A/J and B6 C3H femora had reduced acid phosphate and carbonate levels and an increased collagen crosslink ratio compared to A/J and B6 Modulus values paralleled mineraltomatrix values with A/J femora being the most stiff B6 being the least stiff and C3H having intermediate stiffness In addition worktofailure varied among the strains with the highly mineralized and brittle A/J femora performing the least amount of worktofailure Inbred mice are therefore able to differentially modulate the composition of their bone mineral and the maturity of their bone matrix in conjunction with tissuelevel mechanical properties These results suggest that specific combinations of bone quality and morphological traits are genetically regulated such that mechanically functional bones can be constructed in different waysThe authors thank Damien Laudier for assistance with plastic embedding and the National Institutes of Health NIH for funding support AR44927 AR046121 FTIR images were obtained using the NIHsponsored Core Center AR046121 at the Hospital for Special Surgery

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