Authors: Hermizi Hapidin Faizah Othman Ima Nirwana Soelaiman Ahmad Nazrun Shuid Norazlina Mohamed
Publish Date: 2010/10/16
Volume: 88, Issue: 1, Pages: 41-47
Abstract
Nicotine is a major alkaloid of tobacco which can increase free radical formation leading to osteoporosis The effects of nicotine administration and cessation on bone histomorphometry and biomarkers were studied in 28 Sprague–Dawley male rats Rats aged 3 months and weighing 250–300 g were divided into four groups control C normal saline for 4 months nicotine for 2 months N2 nicotine for 4 months N4 and nicotine cessation NC The NC group was given nicotine for the first 2 months and then allowed to recover for the following 2 months without nicotine Histomorphometric analysis was done using an image analyzer ELISA kits were used to measure serum osteocalcin bone formation marker and pyridinoline PYD bone resorption marker levels at month 0 month 2 and month 4 All test groups showed a significant decrease in BV/TV ObS/BS dLS/BS MAR BFR/BS and osteocalcin levels and an increase in sLS/BS and PYD levels compared to group C No significant differences were observed in all parameters measured among the test groups except for MAR and BFR/BS In conclusion nicotine administration at a dose of 7 mg/kg for 2 and 4 months has detrimental effects on bone metabolism Nicotine administration at 7 mg/kg for 2 months is sufficient to produce significant effects on bone histomorphometric parameters and biomarkers In addition prolonging the treatment for another 2 months did not show any significant differences Cessation of nicotine for 2 months did not reverse the effectsThe authors are grateful to the Ministry of Science Technology and Innovation for funding this research under IRPA grant 060202051EA243 We also express our gratitude to Mr Rafizul Mohd Yusoff Mr Arizi Aziz Mr Faisal Ariffin Ms Hairi Ghazalli Ms Mazliadiyana Mazlan and Ms Azizah Osman for their technical assistance
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