Journal Title
Title of Journal: J Nat Med
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Abbravation: Journal of Natural Medicines
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Authors: Katsuko Komatsu Yohei Sasaki Ken Tanaka Yoshiaki Kuba Hirotoshi Fushimi ShaoQing Cai
Publish Date: 2008/07/17
Volume: 62, Issue: 4, Pages: 413-422
Abstract
Previously Chinese Gajutsu available in Japan was identified from the chloroplast trnK gene sequence to be the rhizomes of Curcuma phaeocaulis and two genotypes of C kwangsiensis Although we defined the two genotypes the pl and gl types on the basis of the nucleotide difference their external features did not correspond to the two phenotypes described in the literature In this paper to investigate the relationship between genotype and phenotype of C kwangsiensis a field investigation was carried out in its main cultivation areas of Guangxi Zhuangzu Autonomous Region and Guangdong Province China and sequence analysis of the trnK gene and singlenucleotide polymorphism SNP analysis of the nuclear 18S rRNA gene were performed on the collected specimens Four genotypes of C kwangsiensis were recognized from the combined 18S rRNA gene–trnK gene sequences homozygoteKglWtk type homozygoteKplZtk type heterozygoteKglWtk type and heterozygoteLtk type Among the four genotypes C kwangsiensis in a field used for cultivation of Gajutsu was of heterozygoteKglWtk type Formation of a heterozygote in the 18S rRNA gene might be a result of crossbreeding of C kwangsiensis with several Curcuma species which had cytosine at nucleotide position 234 GC analysis of the rhizomes revealed that C kwangsiensis was characterized by camphor and βelemene and by detecting additional components such as curdione and curcumenol Curcuma species involved in the formation of the heterozygote might be speculated uponWe are grateful to Mr Morikazu Murakami and to the staff of the Medicinal Plants Research Center of Toyama Prefecture and to Professor Toshio Miyawaki and Dr Hirokazu Kanegane Graduate School of Medicine and Pharmaceutical Sciences University of Toyama for permitting us to use the ABI Prism 310 Genetic Analyzer This work was supported by a GrantinAid for Scientific Research B No 14406030 in 2002–2004 and No 17406004 in 2005–2007 from the Japan Society for the Promotion of Science Takeda Science Foundation and for the 21st Century COE Program from the Ministry of Education Culture Sports Science and Technology Japan
Keywords:
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