Induction of melanogenesis by 4′Emphasis Type="I

Authors: Ippei Horibe Yudai Satoh Yuki Shiota Ayako Kumagai Nanao Horike Hiroshi Takemori Shinichi Uesato Shuji Sugie Katsuyoshi Obata Hidehisa Kawahara Yasuo Nagaoka

Publish Date: 2012/12/04

Volume: 67, Issue: 4, Pages: 705-710

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Abstract

Agents to control melanogenesis are in demand for the development of cosmetics to improve pigmentation disorders of skin and hair In this study we examined and evaluated the effects of flavonoids on melanogenesis in the melanogenic cells model murine B16F10 melanoma cells In the course of this study we found that incubation of the cells in a medium containing 10 μM of the 4′Omethylated flavonoids diosmetin 4′Omethylluteolin acacetin 4′Omethylapigenin or kaempferide 4′Omethylkaempferol increased the melanin contents of the cells 3 to 7fold higher than the control cells The concentrationdependence test revealed that 20 μM acacetin showed the highest effect up to 33fold higher than the vehicle On the other hand the corresponding 4′OHtype flavonoids luteolin apigenin and kaempferol had a significantly smaller effect Furthermore by evaluating the melanogenic proteins we found that the cells treated with 4′Omethylated flavonoids showed higher tyrosinase activity as well as upregulation of tyrosinase expression preceded by activation of cAMP response element binding protein CREB and extracellular signalregulated kinases types 1 and 2 ERK1/2 These results indicate that the 4′Omethyl group of flavonoids plays an important role in the induction of melanogenesis by activating its major signal transduction pathway through the upregulation of phosphoCREB in murine B16F10 melanoma cells

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