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Title of Journal: Res Chem Intermed

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Abbravation: Research on Chemical Intermediates

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Springer Netherlands

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DOI

10.1007/s004770050016

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1568-5675

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Effect of ultraviolet irradiation on photostabilit

Authors: Ye Li Hui Yang Fang Ming Cao
Publish Date: 2009/08/07
Volume: 35, Issue: 5, Pages: 607-613
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Abstract

Application of protein encapsulation silica materials requires biomolecules to maintain stabilization on potentially adverse industrial conditions The Cphycocyanin CPC is chosen as a probe to monitor influence of the protein encapsulation process and to study protein and matrix interaction and stability of protein in silica matrix Both CPCs in solution and in silicate matrix irradiated by ultraviolet rays can result in photodestruction whereas the protein in the silica is less affected The measured photodamage rate constant of CPC in silica is 25 times slower than that of CPC in buffer solution However lifetimes of Cphycocyanin in phosphate buffer and silica matrix are identical These studies suggest that entrapment of CPCs into silica matrixes not only can keep their biological activity and obviously also improve their photostabilityThis project was supported by the National Natural Science Foundation of China Grant No 20873005 Beijing Natural Science Foundation Grant No 2083028 the Scientific Research Foundation for the Returned Overseas Chinese Scholars State Education Ministry and 422 Funds from University of Science and Technology Beijing

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