Authors: ZhaoJian Li Peng Sun HongDi Zhang ShiFang Li Xia Liu RenZhi Wang
Publish Date: 2010/05/12
Volume: 42, Issue: 2, Pages: 255-260
Abstract
Recombinant Semliki Forest virus rSFV as a new kind of neurotropic vector system has great potential of gene therapy for stroke However very little is known about its transduction characteristics in cerebral cortex or corpus callosum CC in vivo which are common targets for gene transfer in experimental stroke therapy Here we investigate and compare rSFVmediated gene expression at above two brain regions in rat 20 × 107 IU of rSFV encoding green fluorescent protein rSFVGFP was locally injected into CC or cerebral cortex in two groups At 36 h following injection the number of GFPpositive cells GFP distribution volume and GFP expression level were examined in the rat brain of each group using continuous frozen sections and enzymelinked immunosorbent assay rSFV vector displayed noticeably different transduction patterns in CC and cerebral cortex in vivo CC injection of vector increased GFPpositive cell number by 802 GFP transduction volume by 958 and GFP expression level by 508 compared with cortical injection all P 001 We concluded that rSFV CC delivery significantly enhances transduction efficiency in rat brain with its ability to achieve transgene extensive transduction and abundant expression and CC may be a favorable target for improving rSFVbased gene delivery efficiency to brain
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