Authors: ZunYi Wang Peiqing Wang Cecilia J Hillard Dale E Bjorling
Publish Date: 2014/11/06
Volume: 55, Issue: 4, Pages: 968-976
Abstract
Endocannabinoids such as Narachidonoylethanolamine AEA also called anandamide exert potent analgesic and antiinflammatory effects Fatty acid amide hydrolase FAAH is primarily responsible for degradation of AEA and deletion of FAAH increases AEA content in various tissues Since FAAH has been shown to be present in the bladder of various species we compared bladder function severity of experimental cystitis and cystitisassociated referred hyperalgesia in male wildtype WT and FAAH knockout KO mice Basal concentrations of AEA were greater and the severity of cyclophosphamide CYPinduced cystitis was reduced in bladders from FAAH KO compared to WT mice Cystitisassociated increased peripheral sensitivity to mechanical stimuli and enhanced bladder activity as reflected by increased voiding frequency were attenuated in FAAH KO compared to WT mice Further abundances of mRNA for several proinflammatory compounds were increased in the bladder mucosa after CYP treatment of WT mice and this increase was inhibited in FAAH KO mice These data indicate that endogenous substrates of FAAH including the cannabinoid AEA play an inhibitory role in bladder inflammation and subsequent changes in pain perception Therefore FAAH could be a therapeutic target to treat clinical symptoms of painful inflammatory bladder diseases
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