Authors: M J MunozDávila G Yagüe M Albert T GarcíaLucas
Publish Date: 2011/07/28
Volume: 31, Issue: 5, Pages: 663-669
Abstract
The performance of Vitek 2 was evaluated for the identification and susceptibility testing of Gramnegative bacilli directly from positive blood cultures bottles Direct inoculation of the positive blood cultures with the Vitek cards IDGN and ASTNO58 was compared with the standard inoculation method based on the subculture of the positive blood culture to agar A total of 142 blood cultures were included in the study of those 119 were from patients’ clinical samples while 23 were artificially prepared with strains showing different mechanisms of resistance A total of 136 958 strains were correctly identified to the species level only 2 14 were misidentified and 4 28 were not identified Susceptibility results were available for all isolates tested against 17 antibiotics thus resulting in a total of 2414 isolate/antimicrobial combinations The error rate was 28 67/2414 overall 06 14/2414 very major errors 01 3/2414 major errors and 21 50/2414 minor errors The direct method detected 885 22/25 of the strains producing extendedspectrum betalactamases ESBLs The susceptibility agreement among the added strains with ESBL AMPc hyperproduction resistance to ceftazidime carbapenems and cefepime was very high Direct identification and susceptibility testing gave rapid and reliable results reducing by 24 h the turnaround time of the microbiology laboratory
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