Authors: B R Cebula J M Rocco J N Maslow V R Irani
Publish Date: 2012/09/19
Volume: 31, Issue: 12, Pages: 3407-3412
Abstract
Mycobacterium avium is an opportunistic pathogen whose pathogenesis is attributed to its serovarspecific glycopeptidolipid ssGPL which varies among its 31 serovars To determine if the presence and type of ssGPLs contribute to M avium pathogenesis we infected murine macrophages mφs with two M avium wild type wt serovars 2 and 8 and their serovarnull strains We examined the influence of ssGPL presence and type on cytokine production in nonactivated −IFNγ and activated +IFNγ mφs and the bacterial intramφ survival over a 6day infection process Serovar2 infections activated TNFα production that increased over the 6 day period and was capable of controlling the intramφ serovar2 null strain In contrast the serovar8 infection stimulated a strong proinflammatory response but was incapable of removing the invading pathogen maybe through IL10 production It was clear that the intracellular growth of serovarnull in contrast to the wt M avium strains was easily controlled Based on our findings and the undisputed fact that M avium ssGPL is key to its pathogenesis we conclude that it is not appropriate to dissect the pathogenesis of one M avium serovar and apply those findings to other serovarsThe authors of this manuscript report no personal or professional relationships which could pose a conflict of interest This research was supported by a Pennsylvania State System of Higher Education PASSHE Faculty Professional Development Council FPDC award to VR Irani 2007 the 2010 Cynthia Sushak Undergraduate Biology Fund for Excellence Award to BR Cebula and JM Rocco and an Indiana University of Pennsylvania IUP School of Graduate Studies and Research SGSR Undergraduate Award to BR Cebula 2010 and JM Rocco 2009
Keywords: