Authors: S S Ahmed E Alp A UluKilic G Dinc Z Aktas B Ada F Bagirova I Baran Y Ersoy S Esen T G Guven J Hopman S Hosoglu F Koksal E Parlak A N Yalcin G Yilmaz A Voss W Melchers
Publish Date: 2016/06/04
Volume: 35, Issue: 9, Pages: 1463-1468
Abstract
Epidemic clones of Acinetobacter baumannii described as European clones I II and III are associated with hospital epidemics throughout the world We aimed to determine the molecular characteristics and genetic diversity between European clones I II and III from Turkey and Azerbaijan In this study a total of 112 bloodstream isolates of carbapenemresistant Acinetobacter spp were collected from 11 hospitals across Turkey and Azerbaijan The identification of Acinetobacter spp using conventional and sensitivity tests was performed by standard criteria Multiplex polymerase chain reaction PCR was used to detect OXA carbapenemaseencoding genes bla OXA23like bla OXA24like bla OXA51like and bla OXA58like Pulsedfield gel electrophoresis PFGE typing was used to investigate genetic diversity The bla OXA51like gene was present in all 112 isolates 75 67 carried bla OXA23like 7 62 carried bla OXA58like genes and 5 45 carried bla OXA24like genes With a 90 similarity cutoff value 15 clones and eight unique isolates were identified The largest clone was cluster D with six subtypes Isolates from clusters D and I were widely spread in seven different geographical regions throughout Turkey However F cluster was found in the northern and eastern regions of Turkey EU clone I was grouped within J cluster with three isolates found in Antalya Istanbul and Erzurum EU clone II was grouped in the U cluster with 15 isolates and found in Kayseri and Diyarbakır The bla OXA24like gene in carbapenemases was identified rarely in Turkey and has been reported for the first time from Azerbaijan Furthermore this is the first multicenter study in Turkey and Azerbaijan to identify several major clusters belonging to European clones I and II of A baumannii
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