Authors: X Wang K E GreenwoodQuaintance M J Karau D R Block J N Mandrekar S A Cunningham J M Mallea R Patel
Publish Date: 2016/10/28
Volume: 36, Issue: 3, Pages: 517-522
Abstract
A relationship between hyperammonemia and Ureaplasma infection has been shown in lung transplant recipients We have demonstrated that Ureaplasma urealyticum causes hyperammonemia in a novel immunocompromised murine model Herein we determined whether Ureaplasma parvum can do the same Male C3H mice were given mycophenolate mofetil tacrolimus and prednisone for 7 days and then challenged with U parvum intratracheally IT and/or intraperitoneally IP while continuing immunosuppression over 6 days Plasma ammonia concentrations were determined and compared using Wilcoxon ranksum tests Plasma ammonia concentrations of immunosuppressed mice challenged IT/IP with spent broth median 188 μmol/L range 102–340 μmol/L were similar to those of normal median 226 μmol/L range 154–284 μmol/L p 005 uninfected immunosuppressed median 231 μmol/L range 122–340 μmol/L p 005 and U parvum IT/IP challenged immunocompetent median 226 μmol/L range 130–330 μmol/L p 005 mice Immunosuppressed mice challenged with U parvum IT/IP median 343 μmol/L range 136–1000 μmol/L or IP median 307 μmol/L range 132–692 μmol/L had higher plasma ammonia concentrations than those challenged IT/IP with spent broth p 0001 U parvum can cause hyperammonemia in pharmacologically immunocompromised miceRP is supported by the National Institutes of Health grant numbers R01 AR056647 and R01 AI91594 XW was supported by the State Scholarship Fund from the China Scholarship Council as a Visiting Scientist at Mayo Clinic The funders played no role in the study design data collection and analysis decision to publish or preparation of the manuscriptThis study was carried out in accordance with recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and was approved by the Mayo Clinic Institutional Animal Care and Use Committee protocol number A8115
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