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Title of Journal: Eur J Clin Microbiol Infect Dis

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Abbravation: European Journal of Clinical Microbiology & Infectious Diseases

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Springer Berlin Heidelberg

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DOI

10.1002/uog.4220

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1435-4373

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Evaluation of phenotypic detection methods for met

Authors: S Peter A Lacher M Marschal F Hölzl M Buhl I Autenrieth M Kaase M Willmann
Publish Date: 2014/01/23
Volume: 33, Issue: 7, Pages: 1133-1141
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Abstract

Metallobetalactamase MBL production in Pseudomonas aeruginosa is a growing issue across the globe Fast and reliable diagnostic tools are needed for appropriate implementation of infection control measures In this study we evaluated the performance of three commercial combined disk tests two EDTA based inhouse combined disk tests and the Carba NP test in comparison to molecular detection of MBL genes on 133 meropenem nonsusceptible nonduplicate P aeruginosa clinical isolates The meropenem/DPA based commercial KPC + MBLconfirm ID kit Rosco Diagnostica Denmark and the MASTDISCS™ ID carbapenemase Enterobacteriaceae detection disc set MAST Diagnostics UK showed sensitivities of 311  and 288  and specificities of 693  and 796  respectively The total MBL confirm kit Rosco Diagnostica Denmark contains imipenem/DPA and imipenem/EDTA combination disks Evaluation of the single disk combinations revealed 844  sensitivity and 818  specificity for the imipenem/DPA assay and 867  sensitivity and 511  specificity for the imipenem/EDTA test Applying both tests simultaneously resulted in a slightly higher sensitivity of 889  but a lower specificity of 489  when compared to the single tests alone The Carba NP test showed 933  sensitivity and 966  specificity All phenotypic combined disk tests lacked either sensitivity or specificity for the detection of MBL in P aeruginosa The Carba NP test showed excellent test properties but suffers from drawbacks in handling and high costs The optimal diagnostic approach needs to be chosen depending on the epidemiological situation laboratory resources and availability of molecular confirmation tests

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