Journal Title
Title of Journal: J Clin Immunol
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Abbravation: Journal of Clinical Immunology
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Authors: Aiping Qi Hu Zhou Zeping Zhou Xin Huang Li Ma Hongmei Wang Yanhui Yang Donglei Zhang Huyuan Li Ruimin Ren Renchi Yang
Publish Date: 2012/12/14
Volume: 33, Issue: 3, Pages: 577-585
Abstract
A total of 237 ITP patients 20 SLE patients and 200 ageand sexmatched healthy controls were included in this study CD4+ CD8+ and CD19+ lymphocytes were purified by magnetic beads sorting from peripheral blood of 37 active chronic ITP patients and 22 ageand sexmatched healthy controls Telomerase activity was assayed by Telo TTAGGG Telomerase PCR ELISA KIT The relative telomere length of peripheral blood mononuclear cell PBMC was measured by a quantitative polymerase chain reactionbased method QPCR from 200 ITP patients and 178 ageand sexmatched healthy controlsTelomerase activity was increased in CD4+ CD8+ and CD19+ lymphocytes from ITP patients compared to those from healthy controls p = 0000 The level of telomerase activity in CD19+ lymphocyte was higher than those in CD4+ and CD8+ lymphocytes Telomerase activity of CD19+ lymphocytes had a modest negative correlation with platelet count in ITP patients p = 0042 The relative telomere length of PBMC in ITP patients was significantly shorter than that in the healthy controls p = 0002 Telomere length of PBMC in active ITP patients was significantly shorter than that in the controls p = 0000 and a tendency to be shorter even in inactive ITP patients p = 0065 Moreover the telomere length in refractory and nonrefractory ITP patients were both significantly shorter than that in the controls p = 0025 p = 0000 However no significant difference in telomere length of PBMC was found between refractory ITP patients and nonrefractory ITP patients p = 0234This work was supported in part by grants of National Natural Science Foundation of China 81070397 81170474 Ministry of Science and Technology 2011ZX0930200704 Ministry of Health 201202017 and Tianjin Municipal Science and Technology Commission 09JCYBJC10900 10JCZDJC19700 The authors would like to thank Prof ManChiu Poon University of Calgary Canada for critical review of the manuscript
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- A Complement Factor B Mutation in a Large Kindred with Atypical Hemolytic Uremic Syndrome
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- Increased T-Cell Activation and Th1 Cytokine Concentrations Prior to the Diagnosis of B-Cell Lymphoma in HIV Infected Patients
- Influence of Maternal Hyperglycemia on IL-10 and TNF-α Production: The Relationship with Perinatal Outcomes
- B-Cell and T-Cell Phenotypes in CVID Patients Correlate with the Clinical Phenotype of the Disease
- First Report on the Moroccan Registry of Primary Immunodeficiencies: 15 Years of Experience (1998–2012)
- Asthma and Hypogammaglobulinemia: an Asthma Phenotype with Low Type 2 Inflammation
- The Histone Peptide H4 71–94 Alone Is More Effective than a Cocktail of Peptide Epitopes in Controlling Lupus: Immunoregulatory Mechanisms
- Histamine Release and Inflammatory Cell Infiltration in Airway Mucosa in Methylene Diphenyl Diisocyanate (MDI)-Induced Occupational Asthma
- Recent Advances in DOCK8 Immunodeficiency Syndrome
- Development of Primary Immunodeficiencies in Africa
- Streptococcal Infections in Patients with Chronic Granulomatous Disease: Case Report and Review of the Literature
- Presenting features and platelet anomalies in WAS: one centre’s experience
- The Effect of Blockade of Tumor Necrosis Factor α on VLA-1+T-Cells in Rheumatoid Arthritis Patients
- Associations Between Autoimmune Thyroid Disease Prognosis and Functional Polymorphisms of Susceptibility Genes, CTLA4 , PTPN22 , CD40 , FCRL3 , and ZFAT , Previously Revealed in Genome-wide Association Studies
- CTLA-4 Gene Exon-1 +49 A/G Polymorphism: Lack of Association with Autoimmune Disease in Patients with Common Variable Immune Deficiency
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