Authors: M S Rahnama’i R Hohnen Ph E V Van Kerrebroeck G A van Koeveringe
Publish Date: 2014/12/06
Volume: 33, Issue: 10, Pages: 1623-1633
Abstract
Nitric oxidestimulated cGMP synthesis represents an important signalling pathway in the urinary bladder Inhibitors of the PDE1 and PDE5 enzyme have been studied to treat storage and voiding disorders in clinical settings The distribution of PDE2 in the bladder is unknown This study focuses on the distribution and site of action of PDE2 within the guinea pig urinary bladder wallSix male guinea pig bladders were dissected and treated in 2 ml Krebs’ solution and 10 µM of the specific PDE2 inhibitor Bay 607550 at 36 °C for 30 min After stimulating tissues with 100 µM of diethylamineNONOate for 10 min the tissues were snap frozen and cut in 10 µm sections which were examined for cGMP immunereactivity costained with either vimentin synaptic vesicle protein 2 calcitonin generelated protein and protein gene product 95PDE2 inhibitor Bay 607550 inhibits cGMP breakdown the most in the urothelial and suburothelial layers as well as on the nerve fibres After inhibition by Bay 607550 cGMP was mainly expressed in the intermuscle interstitial cells and the nerve fibres of the outer muscle layers of lateral wall indicating the presence of PDE2 activity
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