Authors: Julian W Mall Wolfgang Schwenk Andreas W Philipp Rolf Büttemeyer Christian Pollmann
Publish Date: 2003/09/04
Volume: 27, Issue: 10, Pages: 1119-1123
Abstract
The effects of thalidomide after intraperitoneal instillation on the healing of colonic anastomoses are not known A series of 40 New Zealand White rabbits underwent an endtoend colonic anastomosis The animals were randomized into four groups Groups 1 n = 10 and 2 n = 10 were treated with dissolved thalidomide 200 mg/kg intraperitoneally whereas groups 3 n = 10 and 4 n = 10 were treated only with the dissolver Animals were sacrificed at day 3 groups 1 3 and day 7 groups 2 4 Anastomotic healing was tested by measuring the bursting pressure in vitro Immunohistochemical staining of the anastomotic site was performed with polyclonal antibodies against CD31 and Mib1 to determine a possible antiangiogenic or antiproliferative effect Statistical analysis was performed using Spearman’s log rank correlation and paired ttest On postoperative day 3 p 019 and postoperative day 7 p 073 there was no difference in bursting pressure in the treatment and the control groups Angiogenesis scores were reduced at day 3 in the thalidomide group p 005 but did not differ between the groups at day 7 White blood cell counts were decreased in the treatment groups at day 3 p 001 and day 7 compared to control groups p 001 There was no difference in the expression of Mib1 in either group at day 3 or day 7 The intraperitoneal administration of thalidomide does not interfere with the healing of colonic anastomosis Although the angiogenesis score is diminished at day 3 this did not lead to a reduced bursting pressure at day 3 or day 7
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