Authors: Marie Svensson Ling Han Gustav Silfversparre Lena Häggström SvenOlof Enfors
Publish Date: 2004/12/01
Volume: 27, Issue: 2, Pages: 91-97
Abstract
High amounts of outer membrane OM components were released in glucoselimited fedbatch GLFB cultures at 37 °C at specific growth rates approaching 005 h−1 Endotoxin analyses from a 20 °C GLFB culture gave similar results An alternative fermentation technique the temperaturelimited fedbatch TLFB technique reduced the endotoxin concentration in a culture with a biomass concentration of 30 g l−1 from the 850 mg l−1 in traditional GLFB cultures to about 20 mg l−1 The TLFB technique uses the temperature to regulate the dissolved oxygen tension while all substrate components are unregulated It appears to be severe glucose limitation that triggers the extensive release of endotoxins rather than a low growth rate Furthermore it is not the low temperature that stabilizes the OM when using the TLFB technique Simulations and experimental data show that this technique results in the same biomass productivity as the GLFB technique
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