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Title of Journal: Bioprocess Biosyst Eng

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Abbravation: Bioprocess and Biosystems Engineering

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Springer-Verlag

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DOI

10.1016/0020-1383(87)90225-7

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1615-7605

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Effect of compressed fluids treatment on βgalacto

Authors: Anna Rafaela Cavalcante Braga Marceli Fernandes Silva J Vladimir Oliveira Helen Treichel Susana Juliano Kalil
Publish Date: 2012/06/23
Volume: 35, Issue: 9, Pages: 1541-1547
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Abstract

This study aimed at assessing the influence of different pressurized fluids treatment on the enzymatic activity and stability of a lyophilized βgalactosidase The effects of system pressure exposure time and depressurization rate using propane nbutane carbon dioxide and liquefied petroleum gas on the enzymatic activity were evaluated The βgalactosidase activity changed significantly depending on the experimental conditions investigated allowing the selection of the proper compressed fluid for advantageous application of this biocatalyst in enzymatic reactions The residual activity ranged from 321 to 938  after treatment The storage stability of the enzyme after highpressure pretreatment was also monitored and results showed that the biocatalyst activity presents strong dependence of the fluid used in the pretreatment The activity gradually decreases over the time for the enzyme treated with LGP and propane while the enzyme treated with nbutane maintained 96  of its initial activity until 120 days For CO2 there was a reduction of around 40  in the initial activity 90 days of storage The enzyme treated with nbutane also showed a better thermostability in terms of enzymatic halflife

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