Authors: Martín S Iglesias Cynthia Sequeiros Sebastián García Nelda L Olivera
Publish Date: 2017/02/21
Volume: 40, Issue: 6, Pages: 833-842
Abstract
Bacteria from Patagonian Merino wool were isolated to assess their woolkeratinolytic activity and potential for feltresist treatments Strains from Bacillus Exiguobacterium Deinococcus and Micrococcus produced wooldegrading enzymes Bacillus sp G51 showed the highest woolkeratinolytic activity LCMS/MS analysis revealed that G51 secreted two serine proteases belonging to the peptidase family S8 MEROPS and a metalloprotease associated with Bacillolysin along with other enzymes γglutamyltranspeptidase and dihydrolipoyl dehydrogenases that could be involved in reduction of keratin disulfide bonds Optimum pH and temperature of G51 proteolytic activity were 9 and 60 °C respectively More than 80 of activity was retained in H2O2 Triton X100 Tween 20 Lipocol OXO650 Teridol B and βmercaptoethanol Treatment of wool top with G51 enzyme extract caused a decrease in wool felting tendency without significant weight loss 15 Sparse work has so far been performed to investigate suitable keratinases for the organic wool sector This ecofriendly treatment based on a new enzyme combination produced by a wild bacterium has potential for meeting the demands of organic wool processing which bans the use of hazardous chemicals and genetic engineeringThe authors acknowledge to Fuhrmann SA for kindly providing wool tops and to UNILAN Trelew SA Lanera Austral SA Estancia San Guillermo and Estancia Don Martín for raw wool samples The authors are also grateful to Jaime Groizard ALUAR Aluminio Argentino for scanning electron micrographs This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica ANPCyT Ministerio de Ciencia Tecnología e Innovación Productiva Argentina PICT Start Up 2012–2004 and the Consejo Nacional de Investigaciones Científicas y Técnicas CONICET Argentina PIP 11220120100050CO Martín Iglesias is grateful to ANPCyT for his PhD grant
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