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Title of Journal: Bioprocess Biosyst Eng

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Abbravation: Bioprocess and Biosystems Engineering

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Springer Berlin Heidelberg

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DOI

10.1002/elan.201300393

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1615-7605

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Correlation between dielectric property by dielect

Authors: Masaru Hakoda Yusuke Hirota
Publish Date: 2012/11/20
Volume: 36, Issue: 9, Pages: 1219-1227
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Abstract

The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method Our previous studies revealed a correlation between the growth activity and dielectric property ReKω of mouse hybridoma 32H3 cells using dielectrophoretic levitation Furthermore it was clarified that the differentiation activity of many stem cells could be evaluated by the ReKω without differentiation induction In this paper 32H3 cells exposed to an alternating current AC electric field or a direct current DC electric field were cultivated and the influence of damage by the electric field on the growth activity of the cells was examined To evaluate the activity of the cells by measuring the ReKω the correlation between the growth activity and the ReKω of the cells exposed to the electric field was examined The relations between the cell viability growth activity and ReKω in the cells exposed to the AC electric field were obtained The growth activity of the cells exposed to the AC electric field could be evaluated by the ReKω Furthermore it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field

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