Authors: ISon Ng Chiming Ye Zhixiang Zhang Yinghua Lu Keju Jing
Publish Date: 2013/07/05
Volume: 37, Issue: 3, Pages: 415-423
Abstract
Sodium decanoate was first found to be an effective precursor for synthesis of daptomycin from Streptomyces roseosporus NRRL11379 which was increased to 7155fold compared with decanoic acid The optimal flow rate of precursor was at 600 mg/L day after 48 h fermentation From protein analysis via SDSPAGE and identification of Tandem MS/MS afterwards it deciphered that guanosine pentaphosphate synthetase PNPase tripeptidylamino peptidase primarily dealing with daptomycin synthesis By applying Taguchi’s L16 in culture optimization the best yield was obtained from the medium with 60 g/L dextrin 10 g/L dextrose 10 g/L molasses and 8 g/L yeast extract respectively The fedbatch fermentation applied with feedback control of dextrin stimulated the production up to 812 mg/L at 288 h To our best knowledge the daptomycin production in this study is significantly higher than that in previous studies and can make it more widely used in pharmaceutical industryThe authors are grateful to the financial support by the Fundamental Research Funds for the Central Universities 2011121017 the Chinese National Natural Science Foundation 21206141 and the Fujian Provincial Department of Science and Technology 2012I0009
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