Authors: F Cerrone J M Poyatos M MolinaMuñoz C CortésLorenzo J GonzálezLópez B Rodelas
Publish Date: 2012/09/14
Volume: 36, Issue: 7, Pages: 901-910
Abstract
A pilotscale ultrafiltration membrane bioreactor MBR was used for the aerobic treatment of urban wastewater in four experimental stages influenced by seasonal temperature and different sets of operation conditions The structure of the ammoniaoxidizing bacteria AOB community was profiled by temperature gradient gel electrophoresis TGGE based on the amplification and separation of partial ammoniamonoxygenase subunit A amoA genes Canonical correspondence analysis revealed that temperature hydraulic retention time and percentage of ammonia removal had a significant effect on the fingerprints of AOB communities Phylogenetic analysis conducted on amoA/AmoA sequences of reamplified TGGE bands showed however that closely related ammoniaoxidizing populations inhabited the sludge of the MBR in all experimental stages Nitrosomonas cluster 7 populations N europaea–N eutropha cluster prevailed under all conditions tested even when the MBR was operated under complete biomass retention or at low temperatures suggesting that the high ammonia concentrations in the system were determinant to select rstrategist AOBWe thank the Spanish Ministry of Education and Science Ministerio de Educación y Ciencia MEC Spain for funding this study through project reference BES20032350 and for supporting M MolinaMuñoz and J M Poyatos by PhD scholarships PFPI MEC Spain F Cerrone was supported by a Campus World grant funded by the Polytechnic University of Marche Italy The authors thank EMASAGRA for providing their facilities at Estación Depuradora Oeste Puente de los Vados Granada Spain The Instituto de Parasitología y Biología Molecular López Neyra CSIC Granada is acknowledged for assistance at DNA sequencing
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