Authors: Tim Höpfner Arne Bluma Guido Rudolph Patrick Lindner Thomas Scheper
Publish Date: 2009/04/25
Volume: 33, Issue: 2, Pages: 247-256
Abstract
To observe and control cultivation processes optical sensors are used increasingly Important variables for controlling such processes are cell count cell size distribution and the morphology of cells Among turbidity measurement methods imaging procedures are applied for determining these process values A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling On the other hand available imaging inline probes can only deliver a limited number of process values so far This contribution gives an overview of optical procedures for the inline determination of cell count cell size distribution and other variables In particular by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell variables in real time without sampling
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