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Title of Journal: Bioprocess Biosyst Eng

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Abbravation: Bioprocess and Biosystems Engineering

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Springer-Verlag

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DOI

10.1016/0735-1097(95)91693-r

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1615-7605

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Electrochemical recognition for sugars on the chit

Authors: Xiu Zhu Bian Hong Qun Luo Nian Bing Li
Publish Date: 2010/04/06
Volume: 33, Issue: 8, Pages: 971-978
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Abstract

A new amperometric biosensor for the detection of sugars was prepared A glassy carbon electrode was modified with Prussian blue PB nanoparticles protected by chitosan CS and polydiallyldimethylammonium chloride PDDA and then gold nanoparticles were assembled onto the electrode followed by the assembly of 4mercaptophenylboronic acid MPBA onto the surface of gold nanoparticles through a sulfur–Au bond to fabricate a selfassembled biosensor The PB nanoparticles protected by CS and PDDA were characterized using transmission electron microscopy and UV–vis absorption spectroscopy The characterization of the selfassembled electrode was investigated by cyclic voltammetry and electrochemical impedance spectroscopy The pK a values of the MPBA monolayer before and after combining with sugars were determined The fabricated electrode exhibited excellent performances for determining d+glucose d+mannose and d−fructose on the basis of the change in i p of the FeCN 6 3−/4− ion in the presence of sugarsThe authors gratefully acknowledge the support by the National Natural Science Foundation of China no 20575054 China NSFCKorea KOSEF Joint Research Project no 20811140329 and the Municipal Science Foundation of Chongqing City no CSTC2008BB4012 CSTC2008BB4013


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