Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Bioprocess Biosyst Eng

Search In Journal Title:

Abbravation: Bioprocess and Biosystems Engineering

Search In Journal Abbravation:

Publisher

Springer Berlin Heidelberg

Search In Publisher:

DOI

10.1016/0277-5379(83)90322-X

Search In DOI:

ISSN

1615-7605

Search In ISSN:
Search In Title Of Papers:

Thermal inactivation of xanthine oxidase from Emp

Authors: Yuran Zhang Yu Xin Hailin Yang Ling Zhang Xiaole Xia Yanjun Tong Yi Chen Wu Wang
Publish Date: 2013/09/01
Volume: 37, Issue: 4, Pages: 719-725
PDF Link

Abstract

The mechanism of thermal inactivation about xanthine oxidase XOD from Arthrobacter M3 was investigated Results of reducing SDSPAGE indicated that the inactivation of XOD was not related to the peptide degradation Meanwhile fluorimetry and circular dichroism spectroscopy suggested that XOD inactivation might be associated with the exposure of hydrophobic residues to surface and partial loss of secondary structure Specific formation of soluble aggregates of XOD was detected by size exclusion chromatography In addition the thermaldynamic analysis showed that the inactivation kinetics of XOD followed the firstorder model Therefore trehalose cosolute and betaine osmolyte were accordingly employed to attenuate the inactivation of this enzyme The results associated with these two reagents further confirmed that the loss of XOD activity was mainly due to the exposure of hydrophobic residues and formation of aggregation Owing to the added trehalose and betaine halflife could be significantly increased and the inactivation rate constant k was detected as decreasedThis work was supported by Project supported by the National Natural Science Foundation of China Grant No21306064 Natural Science Foundation of Jiangsu Province BK2012119 Program of the Science and Technology Support Plan of Jiangsu Province No BE2010625 and BE2011625 Doctor Candidate Foundation of Jiangnan University JUDCF11012 the Program of Innovation Projects Plan of Jiangsu Province No CXLX11 0502 the Priority Academic Program Development of Jiangsu Higher Education Institutions111 Project No 111206

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Evaluation of a novel Wave Bioreactor ® cellbag for aerobic yeast cultivation
  2. Comparative evaluation of various control schemes for fed-batch fermentation
  3. Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precursor effect and medium optimization
  4. Influence of different chemical pretreatments of elephant grass ( Pennisetum purpureum , Schum.) used as a substrate for cellulase and xylanase production in submerged cultivation
  5. Pretreatment of fibrous biomass and growth of biosurfactant-producing Bacillus subtilis on biomass-derived fermentable sugars
  6. Improvement of acetone, butanol, and ethanol production from woody biomass using organosolv pretreatment
  7. Strategies on process engineering of chondrocyte culture for cartilage tissue regeneration
  8. Newly isolated Bacillus sp. G51 from Patagonian wool produces an enzyme combination suitable for felt-resist treatments of organic wool
  9. Green synthesis of gold nanoparticles using Nyctanthes arbortristis flower extract
  10. Sensitive change of iso-branched fatty acid (iso-15:0) in Bacillus pumilus PAMC 23174 in response to environmental changes
  11. Lipid production by microalgae Chlorella protothecoides with volatile fatty acids (VFAs) as carbon sources in heterotrophic cultivation and its economic assessment
  12. Removal of pigments from molasses wastewater by combining micro-electrolysis with biological treatment method
  13. Small microcapsules of crystal proteins and spores of Bacillus thuringiensis by an emulsification/internal gelation method
  14. Prevalence of Nitrosomonas cluster 7 populations in the ammonia-oxidizing community of a submerged membrane bioreactor treating urban wastewater under different operation conditions
  15. Electrochemical recognition for sugars on the chitosan-poly(diallyldimethylammonium chloride)-nano-Prussian blue/nano-Au/4-mercaptophenylboronic acid modified glassy carbon electrode
  16. Immobilization of Aspergillus niger lipase on chitosan-coated magnetic nanoparticles using two covalent-binding methods
  17. Contribution of microfiltration on phosphorus removal in the sequencing anoxic/anaerobic membrane bioreactor
  18. Influence of flow rate variation on the development of Escherichia coli biofilms
  19. Acetylcholinesterase biosensor for carbaryl detection based on interdigitated array microelectrodes
  20. Oxygen transfer to slurries treated in a rotating drum operated at atmospheric pressure
  21. Transcriptome analysis of xylose metabolism in the thermotolerant methylotrophic yeast Hansenula polymorpha
  22. Simulation of wastewater treatment by aerobic granules in a sequencing batch reactor based on cellular automata
  23. Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field
  24. Batch anaerobic co-digestion of cow manure and waste milk in two-stage process for hydrogen and methane productions
  25. Experiments and three phase modelling of a biofilter for the removal of toluene and trichloroethylene
  26. Effect of natural antioxidants on the lipase activity in the course of batch and continuous glycerolysis of babassu oil
  27. Immobilization of α-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration
  28. Preparation and characterization of chitosan membranes by using a combined freeze gelation and mild crosslinking method
  29. Lignocellulose degrading extremozymes produced by Pichia pastoris : current status and future prospects
  30. A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring
  31. Comparative analysis of solid-state bioprocessing and enzymatic treatment of finger millet for mobilization of bound phenolics
  32. A novel structured bioreactor for solid-state fermentation
  33. Partial nitrification and nutrient removal in intermittently aerated sequencing batch reactors treating separated digestate liquid after anaerobic digestion of pig manure
  34. Escherichia coli β-galactosidase-catalyzed synthesis of 2-phenoxyethanol galactoside and its characterization
  35. Effect of compressed fluids treatment on β-galactosidase activity and stability
  36. Nitrogen removal from slaughterhouse wastewater in a sequencing batch reactor under controlled low DO conditions
  37. Development of a more efficient process for production of fuel ethanol from bamboo
  38. Control of endotoxin release in Escherichia coli fed-batch cultures
Search Result: