Authors: KyungHwan Jung HyangYeol Lee
Publish Date: 2014/09/13
Volume: 38, Issue: 2, Pages: 365-372
Abstract
We synthesized 2phenoxyethanol galactoside PEGal from 2phenoxyethanol PE in which Escherichia coli βgal as E coli cells and lactose were added in the reaction mixture for galactosylation About 40 mM PEGal was maximally synthesized from about 80 mM PE at 24 h as about 50 conversion yield After purifying PEGal the structure of PEGal was identified using LCMS 1H NMR and 13C NMR analyses In addition it was observed that the water solubility of PEGal was increased by galactosylation of PE The MICs of PE and PEGal against Gramnegative and Grampositive bacteria were fairly similar with each other 233–613 mM as the average value PEGal was noticeably less cytotoxic against HACAT cells in particular a remarkable difference in cell viability was observed at concentrations of 20–60 mM PE or PEGal Finally we accomplished the synthesis of less toxic PEGal compared with PE using βgalcontaining E coli cells without changing in the MICs against microorganisms In the future PEGal will be applicable as a substitute for PE as a less toxic preservative for the cosmetic pharmaceutical and food industries
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