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Title of Journal: Bioprocess Biosyst Eng

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Abbravation: Bioprocess and Biosystems Engineering

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Springer-Verlag

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DOI

10.1007/bf01392547

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1615-7605

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Preparation and characterization of chitosan membr

Authors: Carlos E Orrego Jesús S Valencia
Publish Date: 2008/06/11
Volume: 32, Issue: 2, Pages: 197-
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Abstract

When gelification is performed by freezing–thawing repeated cycles the resultant gellike polymer systems are called cryogels This work aims to assess the effect of the addition of glutaraldehyde and 18 Crown Ether6 on surface properties and protein loading of dried chitosan cryogel films Residual water content of treated chitosan membranes ranged between 1193 and 1386 while their water activities vary from 05 to 07 measured from 4 to 60 °C Based on thermal data water evaporation peak and degradation temperatures of chitosan membranes shifted to a higher temperature for crosslinked samples Xray diffractograms provide high values of crystallinity for all the samples 7067–9286 the highest value being for the glutaraldehydetreated membrane Candida rugosa lipase can be immobilized successfully on chitosan membranes Lipase immobilized on glutaraldehydecrosslinked chitosan yielded the highest efficiency in terms of total coupled protein and protein loading efficiencyThe authors are grateful to Dr Alfonso Devia C Laboratorio de Física del Plasma Universidad NacionalManizales for the AFM and XRD data and to Dr Andres Rosales R Laboratorio de Magnetismo Universidad NacionalManizales for performing DSC and TGA analysis

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