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Title of Journal: Bioprocess Biosyst Eng

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Abbravation: Bioprocess and Biosystems Engineering

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Springer Berlin Heidelberg

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1615-7605

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Immobilization of αamylase and amyloglucosidase o

Authors: Kapish Gupta Asim Kumar Jana Sandeep Kumar Mithu Maiti
Publish Date: 2013/04/10
Volume: 36, Issue: 11, Pages: 1715-1724
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Abstract

αAmylase was immobilized on Dowex MAC3 with 88  yield and amyloglucosidase on Amberlite IRA400 ionexchange resin beads with 54  yield by adsorption process Immobilized enzymes were characterized to measure the kinetic parameters and optimal operational parameters Optimum substrate concentration and temperature were higher for immobilized enzymes The thermal stability of the enzymes enhanced after the immobilization Immobilized enzymes were used in the hydrolysis of the natural starch at high concentration 35  w/v The time required for liquefaction of starch to 10 dextrose equivalent DE and saccharification of liquefied starch to 96 DE increased Immobilized enzymes showed the potential for use in starch hydrolysis as done in industry

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