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Title of Journal: Bioprocess Biosyst Eng

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Abbravation: Bioprocess and Biosystems Engineering

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Springer-Verlag

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DOI

10.1007/bf01301625

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1615-7605

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Small microcapsules of crystal proteins and spores

Authors: Karina GarcíaGutiérrez Héctor M PoggyVaraldo Fernando EsparzaGarcía Jorge IbarraRendón Josefina BarreraCortés
Publish Date: 2011/02/23
Volume: 34, Issue: 6, Pages: 701-708
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Abstract

This paper describes a microencapsulation process of a spore crystal aggregate produced by Bacillus thuringiensis var kurstaki HD1 The methodology is based on the emulsification/internal gelation method and was implemented to produce microcapsules of small diameter 10 μm with the capacity to protect the spore crystal aggregate from extreme ultraviolet radiation The diameter of microcapsules was in the range of 31 ± 02–68 ± 04 μm which is considered adequate for biological control purposes The protective effect of the alginate coat was verified by the remaining 60 ± 2 and 40 ± 1 of spore viability and protein activity respectively after UVB radiation of 236 J and with bioassays with Spodoptera frugiperda It is expected that the protective effect of the alginate coat will improve the effectiveness of the BtHD1 formulated as small diameter microcapsules and their yield once they are released into the environment will also be improved

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