Authors: Priscilla F Fonseca Amaral Ana Paula R de Almeida Tathiana Peixoto Maria Helena M RochaLeão João A P Coutinho Maria Alice Z Coelho
Publish Date: 2006/07/16
Volume: 23, Issue: 3, Pages: 339-344
Abstract
The inadequate supply of oxygen to biomass is a critical factor to the productivity of most aerobic submerged fermentations This happens because oxygen is sparingly soluble in the aqueous media The use of a second liquid phase of perfluorocarbon PFC an oxygencarrying compound in the culture medium can increase the availability of oxygen to the microorganisms The effect of perfluorodecalin on Yarrowia lipolytica cultures was investigated in shakeflask cultures It was found that the specific growth rate of Y lipolytica a strictly aerobic yeast increases with increasing PFC concentration Extracellular lipase production was increased with 20 v/v of PFC and agitation of 250 rev/min It was shown that the PFC presence benefitted lipase production and not just its secretion to the extracellular medium
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