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Title of Journal: World J Microbiol Biotechnol

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Abbravation: World Journal of Microbiology and Biotechnology

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Springer Netherlands

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DOI

10.1007/978-1-61779-367-7

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1573-0972

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Assessment of antibacterial activity of silver nan

Authors: Aruna Jyothi Kora J Arunachalam
Publish Date: 2010/09/29
Volume: 27, Issue: 5, Pages: 1209-1216
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Abstract

Antimicrobial activity of silver nanoparticles is gaining importance due its broad spectrum of targets in cell compared to conventional antimicrobial agents In this context a UV photoreduction method was used for the synthesis and the nanoparticles were characterized by UV–Visible spectroscopy transmission electron microscopy atomic force microscopy and thermogravimetric analysis techniques The antibacterial activity of the synthesized silver nanoparticles was evaluated both in liquid and solid growth media employing various susceptibility assays on Pseudomonas aeruginosa a ubiquitous bacterium The dose dependent growth suppression by nanoparticles was studied with well diffusion method By broth dilution method the minimum inhibitory concentration MIC was found to be 2 μg/ml It was observed that the bactericidal effect depends both on nanoparticle concentration and number of bacteria present In our study we could demonstrate the complete antibiofilm activity of silver nanoparticles at a concentration as low as 1 μg/ml Our observations substantiated the association of reactive oxygen species and cell membrane damage in the antibacterial mechanism of silver nanoparticles Our findings suggested that these nanoparticles can be exploited towards the development of potential antibacterial coatings for various biomedical and environmental applications

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