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Title of Journal: World J Microbiol Biotechnol

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Abbravation: World Journal of Microbiology and Biotechnology

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Springer Netherlands

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DOI

10.1002/chin.199825136

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1573-0972

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Effect of the ratio of + and − mating type of

Authors: Maria Varzakakou Triantafyllos Roukas Parthena Kotzekidou
Publish Date: 2010/03/30
Volume: 26, Issue: 12, Pages: 2151-2156
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Abstract

The effect of the ratio of + and − mating type of Blakeslea trispora on carotene production from deproteinized hydrolysed whey in shake flask culture was investigated Also the inoculum ratio of the two mating types on the morphology of the microorganism and the relationship between morphological changes of the fungus and product formation were studied The concentration of carotenes was significantly affected by the ratio of + and − mating type of B trispora A ratio of 110 up to 1100 of + and − type was found to achieve the highest carotene yields The optimum ratio of the + and − mating types for the maximum pigment production 1750 mg/g dry biomass at 8 days of fermentation was found to be 110 The carotene content in B trispora consisted of βcarotene γcarotene and lycopene At the maximum concentration of carotenes the proportion of βcarotene γcarotene and lycopene as percent of total carotenes was 80 12 and 8 respectively Btrispora growing in submerged fermentation is able to develop complex morphologies which have been classified into three major groups freely dispersed hyphae clumps and pellets These parameters are strongly influence the production of carotenesThis paper is part of the 03ED 693 research project implemented within the framework of the Reinforcement Programme of Human Research Manpower PENED and cofinanced by National and Community Funds 25 from the Greek Ministry of DevelopmentGeneral Secretariat of Research and Technology and 75 from EUEuropean Social Fund

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