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Title of Journal: World J Microbiol Biotechnol

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Abbravation: World Journal of Microbiology and Biotechnology

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Kluwer Academic Publishers

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DOI

10.1007/s00023-013-0255-7

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1573-0972

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Immunological Biosensor for Detection of Emphasis

Authors: M K Sharma A K Goel L Singh V K Rao
Publish Date: 2006/03/31
Volume: 22, Issue: 11, Pages: 1155-1159
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Abstract

Environmental surveillance for the presence of Vibrio cholerae O1 is of utmost importance for the effective public health protection of cholera In the present study an amperometric immunosensor was developed for detection of Vibrio cholerae in environmental samples by using disposable screenprinted electrodes SPEs For this purpose the experiments done include fabrication of SPEs by using carbon ink electrochemical characterization of electrodes optimization of dilutions of antibodies and immobilization of antibody V cholerae O1 bacteria were spiked in various environmental water samples in known number The seeded samples were filtered through a 022 µm membrane and the filters enriched in alkaline peptone water for 6 h and then used directly for detection of V cholerae using the immunosensor The immunosensor could detect as few as 8 cfu/ml in handpump water ground water and seawater and 80 cfu/ml in sewer water and tap water The total time taken in this detection assay was 55 min Thus the proposed method is simple and can be used for environmental monitoring of V␣cholerae

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