Authors: Sepideh Hamedi Seyed Abbas Shojaosadati Soheila Shokrollahzadeh Sameereh HashemiNajafabadi
Publish Date: 2013/09/26
Volume: 30, Issue: 2, Pages: 693-704
Abstract
In the present study the biosynthesis of silver nanoparticles AgNPs using Neurospora intermedia as a new nonpathogenic fungus was investigated For determination of biomass harvesting time the effect of fungal incubation period on nanoparticle formation was investigated using UV–visible spectroscopy Then AgNPs were synthesized using both culture supernatant and cellfree filtrate of the fungus Two different volume ratios 1100 and 11 of the culture supernatant to the silver nitrate were employed for AgNP synthesis In addition cellfree filtrate and silver nitrate were mixed in presence and absence of light Smallest average size and highest productivity were obtained when using equal volumes of the culture supernatant and silver nitrate solution as confirmed by UV–visible spectra of colloidal AgNPs Comparing the UV–visible spectra revealed that using cellfree filtrate for AgNP synthesis resulted in the formation of particles with higher stability and monodispersity than using culture supernatant The absence of light in cellfree filtrate mediated synthesis led to the formation of nanoparticles with the lowest rate and the highest monodispersity The presence of elemental silver in all prepared samples was confirmed using EDX while the crystalline nature of synthesized particles was verified by XRD FTIR results showed the presence of functional groups which reduce Ag+ and stabilize AgNPs The presence of nitrate reductase was confirmed in the cellfree filtrate of the fungus suggesting the potential role of this enzyme in AgNP synthesis Synthesized particles showed significant antibacterial activity against E coli as confirmed by examining the growth curve of bacterial cells exposed to AgNPs
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