Authors: Rafaela Coelho Correia Angela Faustino Jozala Kelly Fernanda Martins Thereza Christina Vessoni Penna Eliana Aparecida de Rezende Duek Carlota de Oliveira RangelYagui André Moreni Lopes
Publish Date: 2015/02/13
Volume: 31, Issue: 4, Pages: 649-659
Abstract
The use of polylacticcoglycolic acid PLGA matrix as a biomolecule carrier has been receiving great attention due to its potential therapeutic application In this context we investigated the PLGA matrix capacity to incorporate nisin an antimicrobial peptide capable of inhibiting the growth of Grampositive bacteria and bacterial spores germination Nisinincorporated PLGA matrices were evaluated based on the inhibitory effect against the nisinbioindicator Lactobacillus sakei Additionally the PLGAnisin matrix stability over an 8months period was investigated as well as the nisin release profile For the incorporation conditions we observed that a 5 h incorporation time at 30 °C with 250 μg/mL nisin solution in PBS buffer pH 45 resulted in the highest inhibitory activity of 270 logAU/mL The PLGAnisin matrix was found to be relatively stable and showed sustained drug delivery with continuous release of nisin for 2 weeks Therefore PLGAnisin matrix is could be used as a novel antimicrobial delivery system and an alternative to antibiotics incorporated into PLGA matrices
Keywords: