Authors: Li Min Fan Kamira Barry Geng Dong Hu Shun long Meng Chao Song Wei Wu Jia Zhang Chen Pao Xu
Publish Date: 2015/12/28
Volume: 32, Issue: 1, Pages: 10-
Abstract
The changes of microbial community in aquaculture systems under the effects of stocking densities and seasonality were investigated in tilapia ponds Total DNAs were extracted from the water samples 16S rRNA gene was amplified and the bacterial community analyzed by Illumina highthroughput sequencing obtaining 3486 OTUs from a total read of 715842 sequences Basing on the analysis of bacterial compositions richness diversity bacterial 16S rRNA gene abundance water sample comparisons and existence of specific bacterial taxa within three fish ponds in a 4 months period the study conclusively observed that the dominant phylum in all water samples were similar and they included Proteobacteria Cyanobacteria Bacteroidetes Actinobacteria Planctomycetes and Chlorobi distributed in different proportions in the different months and ponds The seasonal changes had a more pronounced effect on the bacterioplankton community than the stocking densities however some differences between the ponds were more likely caused by feed coefficient than by stocking densities At the same time most bacterial communities were affected by the nutrient input except phylum Cyanobacteria that was also affected by the feed control of tilapia
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