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Title of Journal: World J Microbiol Biotechnol

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Abbravation: World Journal of Microbiology and Biotechnology

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Springer Netherlands

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DOI

10.1002/chin.199450088

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1573-0972

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Comparison of 16S rRNA sequencing with biochemical

Authors: Arij Mechergui Wafa Achour Assia Ben Hassen
Publish Date: 2014/03/18
Volume: 30, Issue: 8, Pages: 2181-2188
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Abstract

We aimed to compare accuracy of genus and species level identification of Neisseria spp using biochemical testing and 16S rRNA sequence analysis These methods were evaluated using 85 Neisseria spp clinical isolates initially identified to the genus level by conventional biochemical tests and API NH system BioMérieux® In 34  29/85 more than one possibility was given by 16S rRNA sequence analysis In 6  5/85 one of the possibilities offered by 16S rRNA gene sequencing agreed with the result given by biochemical testing In 4  3/85 the same species was given by both methods 16S rRNA gene sequencing results did not correlate well with biochemical tests

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