Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Plant Mol Biol

Search In Journal Title:

Abbravation: Plant Molecular Biology

Search In Journal Abbravation:

Publisher

Kluwer Academic Publishers

Search In Publisher:

DOI

10.1016/0049-3848(93)90303-6

Search In DOI:

ISSN

1573-5028

Search In ISSN:
Search In Title Of Papers:

Emphasis Type="Italic"RTS/Emphasis a rice ant

Authors: Hong Luo JangYong Lee Qian Hu Kimberly NelsonVasilchik Timothy K Eitas Colin Lickwar Albert P Kausch Joel M Chandlee Thomas K Hodges
Publish Date: 2006/08/01
Volume: 62, Issue: 3, Pages: 397-408
PDF Link

Abstract

A tapetumspecific gene RTS has been isolated by differential screening of a cDNA library from rice panicles RTS is a unique gene in the rice genome RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther’s tapetum during meiosis and disappears before anthesis RTS has no introns and encodes a putative polypeptide of 94 amino acids with a hydrophobic Nterminal region The nucleotide and deduced amino acid sequence of the gene do not show significant homology to any known sequences However a sequence in the promoter region GAATTTGTTA differs only by one or two nucleotides from one of the conserved motifs in the promoter region of two pollenspecific genes of tomato Several other sequence motifs found in other antherspecific promoters were also identified in the promoter of the RTS gene Transgenic and antisense RNA approaches revealed that RTS gene is required for male fertility in rice The promoter region of RTS when fused to the Bacillus amyloliquefaciens ribonuclease gene barnase or the antisense of the RTS gene is able to drive tissuespecific expression of both genes in rice creeping bentgrass Agrostis stolonifera L and Arabidopsis conferring male sterility to the transgenic plants Light and nearinfrared confocal microscopy of crosssections through developing flowers of malesterile transgenics shows that tissuespecific expression of barnase or the antisense RTS genes interrupts tapetal development resulting in deformed nonviable pollen These results demonstrate a critical role of the RTS gene in pollen development in rice and the versatile application of the RTS gene promoter in directing antherspecific gene expression in both monocotyledonous and dicotyledonous plants pointing to a potential for exploiting this gene and its promoter for engineering male sterility for hybrid production of various plant speciesThis work was supported by grants from the Rockefeller foundation to Purdue University TKH and in part by the USDASBIR Grants 20033361013107 20053361015620 to HybriGene Inc HL and by the USDABRAG grant 20053945416511 to Clemson University HL Technical Contribution No 5214 of the Clemson University Experiment Station

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula
  2. RNA processing in plant mitochondria is independent of transcription
  3. A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)
  4. Overexpression of Arabidopsis damaged DNA binding protein 1A (DDB1A) enhances UV tolerance
  5. Functional analysis of Arabidopsis genes involved in mitochondrial iron–sulfur cluster assembly
  6. Camelid nanobodies with high affinity for broad bean mottle virus : a possible promising tool to immunomodulate plant resistance against viruses
  7. Genome-Wide Identification of microRNAs and Their Targets in Cold-Stored Potato Tubers by Deep Sequencing and Degradome Analysis
  8. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides
  9. Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
  10. Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice
  11. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  12. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  13. Transcriptional Regulation of the Rice Phytase Gene OsPHY1 by Several Phytohormones and Osmotic Stresses Using Promoter-GUS Analysis
  14. Production of hyperthermostable GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants enables complete hydrolysis of methylglucuronoxylan to fermentable sugars for biofuel production
  15. Constitutive and stress-inducible overexpression of a native aquaporin gene ( MusaPIP2;6 ) in transgenic banana plants signals its pivotal role in salt tolerance
  16. Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels
  17. Salt-induced expression of genes related to Na + /K + and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species
  18. Salt-induced expression of genes related to Na + /K + and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species
  19. Plant root-microbe communication in shaping root microbiomes
  20. Wheat Neocentromeres Found in F1 Triticale × Tritordeum Hybrids (AABBRH ch ) After 5-Azacytidine Treatment
  21. Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector
  22. BcJMJ30 , the Gene Encoding jmjC Domain-Containing Histone Demethylase Is Associated with Pollen Development and Fertilization in Brassica campestris ssp. chinensis
  23. Two MYB-related transcription factors play opposite roles in sugar signaling in Arabidopsis
  24. Differential Protein Phosphorylation Regulates Chloroplast Movement in Response to Strong Light and Darkness in Arabidopsis thaliana
  25. Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
  26. Self-compatible peach ( Prunus persica ) has mutant versions of the S haplotypes found in self-incompatible Prunus species
  27. Sulfite Oxidase is Essential for Timely Germination of Maize Seeds upon Sulfite Exposure
  28. hATpin , a Family of MITE-like hAT Mobile Elements Conserved in Diverse Plant Species that Forms Highly Stable Secondary Structures
  29. Molecular Cloning, Biochemical Characterization, and Differential Expression of an Acetyl-CoA C-Acetyltransferase Gene ( AACT ) of Brahmi ( Bacopa monniera )
  30. A residue substitution in the plastid ribosomal protein L12/AL1 produces defective plastid ribosome and causes early seedling lethality in rice
  31. Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco
  32. A pFBP6::Barnase Construct Resulted in Stigma and Style Ablation and Floral Abscission in Transgenic Tobacco
  33. Genome-wide analysis of S- Locus F-box-like genes in Arabidopsis thaliana
  34. Enhanced cloning efficiency of differential display PCR amplicons
  35. Virus-Induced Gene Silencing of a Putative Glutathione S -Transferase Gene Compromised Ol-1 -Mediated Resistance against Powdery Mildew in Tomato
  36. Characterization, expression and function of DORMANCY ASSOCIATED MADS-BOX genes from leafy spurge
  37. Novel interaction between CCR4 and CAF1 in rice CCR4–NOT deadenylase complex
  38. Flow Karyotyping of Wheat Addition Line “T240” with a Haynaldia villosa 6VS Telosome
  39. Expression Analysis of Fatty Acid Biosynthetic Pathway Genes during Interactions of Oil Palm ( Elaeis guineensis Jacq.) with the Pathogenic Ganoderma boninense and Symbiotic Trichoderma harzianum Fungal Organisms
  40. Small RNA and Degradome Deep Sequencing Reveals Peanut MicroRNA Roles in Response to Pathogen Infection
  41. Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants
  42. EST database for early flower development in California poppy ( Eschscholzia californica Cham., Papaveraceae) tags over 6000 genes from a basal eudicot
  43. Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus , Tomato spotted wilt virus and Potato virus X
  44. A novel shaggy-like kinase interacts with the Tomato leaf curl virus pathogenicity determinant C4 protein
  45. Isolation and amplification of DNA from rhizomes of turmeric and ginger
  46. A Quantitative Method to Monitor the Efficacy of Inhibitors Against the Chymotrypsin-Like Activity of the Proteasome in Tobacco Leaf Protoplasts
  47. SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana
  48. Phylogenetic Analysis of S -Locus Genes Reveals the Complicated Evolution Relationship of S Haplotypes in Brassica
  49. QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat ( Triticum aestivum L. )
  50. Expression Profile of Early Responsive Genes Under Salt Stress in Upland Cotton ( Gossypium hirsutum L.)
  51. Overproduction of stromal ferredoxin:NADPH oxidoreductase in H 2 O 2 -accumulating Brassica napus leaf protoplasts
  52. Artificial microRNA mediated gene silencing in plants: progress and perspectives
  53. A Novel Co-immunoprecipitation Protocol Based on Protoplast Transient Gene Expression for Studying Protein–protein Interactions in Rice
  54. Cloning and Expression Analysis of Wheat Pheophorbide a Oxygenase Gene TaPaO
  55. BAHD superfamily of acyl-CoA dependent acyltransferases in Populus and Arabidopsis : bioinformatics and gene expression
  56. Expression of Aprotinin in Anther Causes Male Sterility in Tobacco var Petit havana
  57. Transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions
  58. Highly Conserved UFD1 Proteins Among Eukaryotes Exhibit Considerable C-Terminus Diversity in Different Taxa
  59. Isolation of cDNAs encoding for two distinct isoforms of the TATA-Box binding proteins from common bean ( Phaseolus vulgaris L.)
  60. Dwarf 88 , a novel putative esterase gene affecting architecture of rice plant
  61. Identification of a soybean chloroplast DNA replication origin-binding protein
Search Result: