Authors: Ana Paula Guedes Frazzon Melissa V Ramirez Ujwala Warek Janneke Balk Jeverson Frazzon Dennis R Dean Brenda S J Winkel
Publish Date: 2007/04/07
Volume: 64, Issue: 3, Pages: 225-240
Abstract
Machinery for the assembly of the iron–sulfur Fe–S clusters that function as cofactors in a wide variety of proteins has been identified in microbes insects and animals Homologs of the genes involved in Fe–S cluster biogenesis have recently been found in plants as well and point to the existence of two distinct systems in these organisms one located in plastids and one in mitochondria Here we present the first biochemical confirmation of the activity of two components of the mitochondrial machinery in Arabidopsis AtNFS1 and AtISU1 Analysis of the expression patterns of the corresponding genes as well as AtISU2 and AtISU3 and the phenotypes of plants in which these genes are up or downregulated are consistent with a role for the mitochondrial Fe–S assembly system in the maturation of proteins required for normal plant development
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