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Title of Journal: Plant Mol Biol

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Abbravation: Plant Molecular Biology

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Springer Netherlands

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DOI

10.1016/0024-3205(79)90337-0

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ISSN

1573-5028

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Production of hyperthermostable GH10 xylanase Xyl1

Authors: Jae Yoon Kim Musa Kavas Walid M Fouad Guang Nong James F Preston Fredy Altpeter
Publish Date: 2010/11/16
Volume: 76, Issue: 3-5, Pages: 357-369
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Abstract

Overcoming the recalcitrance in lignocellulosic biomass for efficient hydrolysis of the polysaccharides cellulose and hemicellulose to fermentable sugars is a research priority for the transition from a fossilfuelbased economy to a renewable carbohydrate economy Methylglucuronoxylans MeGXn are the major components of hemicellulose in woody biofuel crops Here we describe efficient production of the GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants and demonstrate exceptional stability and catalytic activities of the in planta produced enzyme Fully expanded leaves from homotransplastomic plants contained enzymatically active Xyl10B at a level of 11–15 of their total soluble protein Transplastomic plants and their seed progeny were morphologically indistinguishable from nontransgenic plants Catalytic activity of in planta produced Xyl10B was detected with poplar sweetgum and birchwood xylan substrates following incubation between 40 and 90°C and was also stable in dry and stored leaves Optimal yields of Xyl10B were obtained from dry leaves if crude protein extraction was performed at 85°C The transplastomic plant derived Xyl10B showed exceptional catalytic activity and enabled the complete hydrolysis of MeGXn to fermentable sugars with the help of a single accessory enzyme αglucuronidase as revealed by the sugar release assay Even without this accessory enzyme the majority of MeGXn was hydrolyzed by the transplastomic plantderived Xyl10B to fermentable xylose and xylobioseThe authors like to thank the Consortium of Plant Biotechnology University of Florida SNRE University of Florida Research Opportunity Fund and Florida Energy Systems Consortium for partial financial support Jae Yoon Kim was partially supported by a National Research Foundation of Korea NRF grant from the Korean government MEST KRF2007357F00001 Musa Kavas was partially supported by a grant from the Scientific and Technological Research Council of Turkey

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