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Title of Journal: J SolGel Sci Technol

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Abbravation: Journal of Sol-Gel Science and Technology

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Springer US

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DOI

10.1007/s11104-011-1050-1

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1573-4846

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Bioencapsulation of living yeast Emphasis Type="

Authors: Chunfeng Guan Gang Wang Jing Ji Jiehua Wang Haiyong Wang Ming Tan
Publish Date: 2008/07/21
Volume: 48, Issue: 3, Pages: 369-377
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Abstract

Bioencapsulation is an attractive way to immobilize biomolecules including cells in silica or hybrid materials Until now the sol–gel technology was preferentially utilized in bacteria or yeast cells immobilization Biomimetic route to silica materials synthesis offers considerable advantages over conventional strategies Recently lysozyme has been used to induce the rapid synthesis of amorphous silica and it provides a convenient approach to synthesize silica under ambient temperature and pH In this study we showed that Pichia pastoris can be transformed with the gene encoding the losozyme The heterologously produced lysozyme was enzymatically active which was confirmed by measuring the bactericidal activity against Grampositive bacteria and staining with Rhodamine 123 formation of silica from silicic acid Scanning electron microscopic analysis revealed that a viscous silica cover formed around the yeast in the presence of silicic acid Furthermore we demonstrated that the growth kinetics of P pastoris was not affected after transformation when growing in the medium with silicic acid In conclusion yeast cells can be bioengineered to secrete lysozyme which in turn conveniently mediate the encapsulation of yeast cells within silica The facile method for yeast encapsulation may prove a novel way for cell immobilization through the combination of genetic approach and biomineralization

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Other Papers In This Journal:
  1. Structure–property tuning in hydrothermally stable sol–gel-processed hybrid organosilica molecular sieving membranes
  2. Physical–chemical explanation of fire-fighting efficiency of FHF (fast-hardening foam) based on structured silica particles
  3. Preparation and characterization of epoxy–silica networks chemically bonded through aminophenyl-trimethoxysilane
  4. Biosynthesis of silver nanoparticles using Lantana camara flower extract and its application
  5. Structure, photoluminescence and electrical properties of Eu–Nd codoped CaBi 4 Ti 4 O 15 synthesized by sol–gel method
  6. Influences of preferred orientation growth on electrical properties of ZnO:Al films by sol–gel method
  7. Synthesis of nanosized carbonated apatite by a modified Pechini method: hydroxyapatite nucleation from a polymeric matrix
  8. Effect of excess methanol addition on the morphology of sonogel-derived silica
  9. Removal of Pb(II) ions from aqueous systems using thiol-functionalized cobalt-ferrite magnetic nanoparticles
  10. Simple synthesis of Co 3 O 4 nanoflakes using a low temperature sol–gel method suitable for photodegradation of dyes
  11. The effect of PO 2,5 and AlO 1,5 additions on structural changes and crystallization behavior of SiO 2 -TiO 2 sol-gel derived glasses and thin films
  12. Antiwetting silica–gelatin nanohybrid and transparent nano coatings synthesised through an aqueous sol–gel process
  13. Comparison of structure and phase change characteristic of microencapsulated core/shell Al–Si alloy microparticles synthesized by two methods
  14. Optical properties of yellow-light-emitting LiZnVO 4 :Eu 3+ phosphor prepared by sol–gel method
  15. CuAl 2 O 4 powder synthesis by sol-gel method and its photodegradation property under visible light irradiation
  16. GPS patch antenna performance by modification of Zn (1−x) Ca x Al 2 O 4 -based microwave dielectric ceramics
  17. Effect of thermal annealing on Nd 2 O 3 -doped silica powder prepared by the solgel process
  18. Effect of sulphate groups on catalytic properties of chromium supported by zirconia in the n -hexane aromatization
  19. Synthesis of LiCoO 2 by l -apple acid assisted sol–gel method and its electrochemical behavior in aqueous lithium-ion battery
  20. Investigation of Br–N Co-doped TiO 2 photocatalysts: preparation and photocatalytic activities under visible light
  21. Optimization of process variables and corrosion properties of a multi layer silica sol gel coating on AZ91D using the Box–Behnken design
  22. Al 2 O 3 /TiO 2 core/shell powder derived by novel sol–gel routes
  23. A new surface modification method to improve the dispersity of nano-silica in organic solvents
  24. Synthesis of BiFeO 3 nanoparticles with small size
  25. Sustained release drug delivery using supramolecular hydrogels of the triblock copolymer PCL–PEG–PCL and α-cyclodextrin
  26. A novel lariat - silacrown ether based in sol–gel process: synthesis, characterization and interaction study with the copper(II) ion
  27. Sol–gel doped TiO 2 nanomaterials: a comparative study
  28. Hysteresis-free, energy efficient twisted nematic liquid crystal systems based on IB-irradiated gallium-doped tin oxide films
  29. The preparation and characterization graphene-cross-linked phenol–formaldehyde hybrid carbon xerogels
  30. Preparation and characterization of silica-titania aerogel-like balls by ambient pressure drying
  31. Phosphorus-doped organic–inorganic hybrid silicon coating for improving fire retardancy of polyacrylonitrile fabric
  32. Synthesis and characterization of hollow LiNiO 2 fibers via sol-electrospinning method
  33. Improvement of electrical properties of sol–gel derived ZnO:Ga films by infrared heating method
  34. Bioactive textiles by sol–gel immobilised natural active agents
  35. Sol–gel synthesis, characterization and study of substitution effects in different gallium-containing garnets
  36. Ni-based xero- and aerogels as catalysts for nitroxidation processes
  37. A spectroscopic study of calcium aluminate gels obtained from aluminium sec- butoxide chelated with ethyl acetoacetate in various ratios
  38. Promotion of electrochemical performance by tailoring the surface of β-Ni(OH) 2 nanosheets
  39. Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules
  40. Characterization of nanostructured magnetite thin films produced by sol–gel processing
  41. Structural characterisation of a sol-gel copolymer synthesised from aliphatic and aromatic alkoxysilanes using 29 Si-NMR spectroscopy
  42. Preparation and electrochemical properties of V 2 O 5 submicron-belts synthesized by a sol–gel H 2 O 2 route
  43. Physico-chemical properties of ambiently dried sodium silicate based aerogels catalyzed with various acids
  44. Effect of 3-glycidoxypropyltrimethoxysilane precursor on the properties of ambient pressure dried silica aerogels
  45. Synthesis of garnet structured Li 7+ x La 3 Y x Zr 2- x O 12 ( x = 0–0.4) by modified sol–gel method
  46. Phase-separation and distribution of phenyl groups for PhTES-TEOS coatings prepared on polycarbonate substrate
  47. Comparative analysis of NdCaCoO 4 phase formation from cryogel and from solid state precursors
  48. Notable enlargement of remnant polarization for fatigue-free SrBi 4 Ti 4 O 15 thin films by La-substitution
  49. Preparation and characterisation of NdAlO 3 nanocrystals by modified sol–gel method
  50. Optical and structural characteristics of yttrium doped ZnO films using sol–gel technology
  51. Proteins conjugation with ZnO sol–gel nanopowders
  52. Photocatalytic degradation of indigo carmine using Nd-doped TiO 2 -decorated graphene oxide nanocomposites
  53. Small-angle X-ray scattering from wet gels prepared from co-hydrolysis of tetraethoxysilane and vinyltriethoxysilane
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