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Title of Journal: J SolGel Sci Technol

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Abbravation: Journal of Sol-Gel Science and Technology

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Springer US

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DOI

10.1016/0021-9681(77)90074-1

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1573-4846

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Proteins conjugation with ZnO sol–gel nanopowders

Authors: Lidia Armelao Gregorio Bottaro Laura Bovo Chiara Maccato Eugenio Tondello Francesca Anselmi Sara Bersani Paolo Caliceti
Publish Date: 2011/06/09
Volume: 60, Issue: 3, Pages: 352-358
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Abstract

The conjugation between probe biomolecules and inorganic nanoparticles has been studied Three different and biologically relevant proteins bovine serum albumin BSA lysozyme LSZ and Ribonuclease A RNAseA have been selected as model systems because of their difference in size and isoelectric point Zinc oxide nanoparticles synthesized via sol–gel have been thoroughly characterized by Xray Photoelectron Spectroscopy Scanning Electron Microscopy and Xray Diffraction and subsequently used as platforms for immobilization of the biomolecules The interaction of the three proteins with the ZnO surface was performed in phosphate buffer solutions at pH 72 in order to mimic physiological fluids and was investigated through fluorescence experiments The obtained results indicate that conjugation of BSA LZS and RNAseA on the oxide nanoparticles was mostly dictated by the overall charge of the different proteins Electrostatic bonds dominate the formation of the protein/ZnO conjugates whereas the size of the proteins seems to play a negligible role under the adopted experimental conditionsThe research was supported by Italian MIUR through the FIRB RBPR05JH2P “Rete ItalNanoNet” Project MSE Industria 2015 ALADIN Progetto Strategico HELIOS Università di Padova and PRINCOFIN 2008 Mr Antonio Ravazzolo ISTM CNR University of Padova is gratefully acknowledged for technical assistance

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  2. Physical–chemical explanation of fire-fighting efficiency of FHF (fast-hardening foam) based on structured silica particles
  3. Preparation and characterization of epoxy–silica networks chemically bonded through aminophenyl-trimethoxysilane
  4. Biosynthesis of silver nanoparticles using Lantana camara flower extract and its application
  5. Structure, photoluminescence and electrical properties of Eu–Nd codoped CaBi 4 Ti 4 O 15 synthesized by sol–gel method
  6. Influences of preferred orientation growth on electrical properties of ZnO:Al films by sol–gel method
  7. Synthesis of nanosized carbonated apatite by a modified Pechini method: hydroxyapatite nucleation from a polymeric matrix
  8. Effect of excess methanol addition on the morphology of sonogel-derived silica
  9. Removal of Pb(II) ions from aqueous systems using thiol-functionalized cobalt-ferrite magnetic nanoparticles
  10. Simple synthesis of Co 3 O 4 nanoflakes using a low temperature sol–gel method suitable for photodegradation of dyes
  11. The effect of PO 2,5 and AlO 1,5 additions on structural changes and crystallization behavior of SiO 2 -TiO 2 sol-gel derived glasses and thin films
  12. Antiwetting silica–gelatin nanohybrid and transparent nano coatings synthesised through an aqueous sol–gel process
  13. Comparison of structure and phase change characteristic of microencapsulated core/shell Al–Si alloy microparticles synthesized by two methods
  14. Bioencapsulation of living yeast ( Pichia pastoris ) with silica after transformation with lysozyme gene
  15. Optical properties of yellow-light-emitting LiZnVO 4 :Eu 3+ phosphor prepared by sol–gel method
  16. CuAl 2 O 4 powder synthesis by sol-gel method and its photodegradation property under visible light irradiation
  17. GPS patch antenna performance by modification of Zn (1−x) Ca x Al 2 O 4 -based microwave dielectric ceramics
  18. Effect of thermal annealing on Nd 2 O 3 -doped silica powder prepared by the solgel process
  19. Effect of sulphate groups on catalytic properties of chromium supported by zirconia in the n -hexane aromatization
  20. Synthesis of LiCoO 2 by l -apple acid assisted sol–gel method and its electrochemical behavior in aqueous lithium-ion battery
  21. Investigation of Br–N Co-doped TiO 2 photocatalysts: preparation and photocatalytic activities under visible light
  22. Optimization of process variables and corrosion properties of a multi layer silica sol gel coating on AZ91D using the Box–Behnken design
  23. Al 2 O 3 /TiO 2 core/shell powder derived by novel sol–gel routes
  24. A new surface modification method to improve the dispersity of nano-silica in organic solvents
  25. Synthesis of BiFeO 3 nanoparticles with small size
  26. Sustained release drug delivery using supramolecular hydrogels of the triblock copolymer PCL–PEG–PCL and α-cyclodextrin
  27. A novel lariat - silacrown ether based in sol–gel process: synthesis, characterization and interaction study with the copper(II) ion
  28. Sol–gel doped TiO 2 nanomaterials: a comparative study
  29. Hysteresis-free, energy efficient twisted nematic liquid crystal systems based on IB-irradiated gallium-doped tin oxide films
  30. The preparation and characterization graphene-cross-linked phenol–formaldehyde hybrid carbon xerogels
  31. Preparation and characterization of silica-titania aerogel-like balls by ambient pressure drying
  32. Phosphorus-doped organic–inorganic hybrid silicon coating for improving fire retardancy of polyacrylonitrile fabric
  33. Synthesis and characterization of hollow LiNiO 2 fibers via sol-electrospinning method
  34. Improvement of electrical properties of sol–gel derived ZnO:Ga films by infrared heating method
  35. Bioactive textiles by sol–gel immobilised natural active agents
  36. Sol–gel synthesis, characterization and study of substitution effects in different gallium-containing garnets
  37. Ni-based xero- and aerogels as catalysts for nitroxidation processes
  38. A spectroscopic study of calcium aluminate gels obtained from aluminium sec- butoxide chelated with ethyl acetoacetate in various ratios
  39. Promotion of electrochemical performance by tailoring the surface of β-Ni(OH) 2 nanosheets
  40. Improved silica xerogel film processing for MALDI-TOF–MS quantitative analysis of peptides and small molecules
  41. Characterization of nanostructured magnetite thin films produced by sol–gel processing
  42. Structural characterisation of a sol-gel copolymer synthesised from aliphatic and aromatic alkoxysilanes using 29 Si-NMR spectroscopy
  43. Preparation and electrochemical properties of V 2 O 5 submicron-belts synthesized by a sol–gel H 2 O 2 route
  44. Physico-chemical properties of ambiently dried sodium silicate based aerogels catalyzed with various acids
  45. Effect of 3-glycidoxypropyltrimethoxysilane precursor on the properties of ambient pressure dried silica aerogels
  46. Synthesis of garnet structured Li 7+ x La 3 Y x Zr 2- x O 12 ( x = 0–0.4) by modified sol–gel method
  47. Phase-separation and distribution of phenyl groups for PhTES-TEOS coatings prepared on polycarbonate substrate
  48. Comparative analysis of NdCaCoO 4 phase formation from cryogel and from solid state precursors
  49. Notable enlargement of remnant polarization for fatigue-free SrBi 4 Ti 4 O 15 thin films by La-substitution
  50. Preparation and characterisation of NdAlO 3 nanocrystals by modified sol–gel method
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  52. Photocatalytic degradation of indigo carmine using Nd-doped TiO 2 -decorated graphene oxide nanocomposites
  53. Small-angle X-ray scattering from wet gels prepared from co-hydrolysis of tetraethoxysilane and vinyltriethoxysilane
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