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Title of Journal: Indian J Pediatr

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Abbravation: The Indian Journal of Pediatrics

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Springer-Verlag

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DOI

10.1016/j.aorn.2007.04.006

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0973-7693

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Emphasis Type="Italic"Staphylococcus aureus/Emp

Authors: KV Ramana SK Mohanty CG Wilson
Publish Date: 2009/06/26
Volume: 76, Issue: 8, Pages: 813-
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Abstract

Nasal swabs taken for S aureus in 392 healthy school going children aged between 5 and 15 yr belonging to three schools surrounding Kamineni Institute of Medical Sciences Narketpally Andhra Pradesh India Swabs were inoculated in to Mannitol Salt Agar MSA and incubation at 37° C for 24hr Staphylococcus aureus isolates were identified by standard microbiological methods such as Gram’s stain catalase and coagulase MICs were determined by Agar dilution technique against Vancomycin Ciprofloxacin Sparfloxacin Sparfloxacin β Cyclodextrin Antimicrobial resistance patterns of all the isolates against Oxacillin 1 μg Penicillin 10units Ampicillin 10μg Cotrimoxazole 2375 μg Erythromycin 15 μg Tetracycline 30 μg and Gentamicin 10 μg were tested using KirbyBaeur disk diffusion method in accordance with CLSI standardsOf the 392 samples screened 6316 showed the growth of Staphylococcus aureus 1219 isolates were found to be MRSA Antimicrobial susceptibility testing using Agar dilution method against Vancomycin ciprofloxacin Sparfloxacin and Sparfloxacin β Cyclodextrin revealed MICs in the range of 052 μg/ml 051 μg/ml 05 μg/ml and 00312–0250 μg/ml respectively Disk diffusion method showed that all the isolates were resistant to Penicillin and Ampicillin A resistance of 143 25 and 222 was observed against Cotrimoxazole Erythromycin and Tetracycline respectively Gentamiicin was the only antibiotic against which most of the isolates were sensitive

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