Authors: Patricia M Davidson Olivia Fromigué Pierre J Marie Vasif Hasirci Günter Reiter Karine Anselme
Publish Date: 2009/12/10
Volume: 21, Issue: 3, Pages: 939-946
Abstract
Osteosarcomaderived cell lines SaOs2 MG63 have recently been shown to deform their nucleus considerably in response to surface topography Such a deformation had not been described previously Here we present results on additional cell lines including cancerous OHS4 U2OS immortalized F/STRO1+A and FHSO6 and healthy cells HOP The cancerous cells were found to deform extensively the immortalized cells showed small deformations whereas the healthy cells showed deformation only at short incubation times These results suggest a strong link between the malignant transformation of cells and the state of the cytoskeletal network We propose mechanisms to explain the deformation in which the cytoskeleton either pushes down on the nucleus during spreading or pulls it down upon adhesion to the pillarsFinancial support provided through the European Community’s ‘‘Marie Curie Actions’’ under contract MRTNCT2004005516 BioPolySurf V H also acknowledges the support of TUBITAK the Scientific and Technical Research Council of Turkey through the project Nanobiomat TBAG 105T508 We also acknowledge Prof A Aydinli of Bilkent University for the patterned templates
Keywords: