Authors: Ali Fattahi Mohammadhossein Asgarshamsi Farshid Hasanzadeh Jaleh Varshosaz Mahbobeh Rostami Mina Mirian Hojjat Sadeghialiabadi
Publish Date: 2015/02/13
Volume: 26, Issue: 2, Pages: 119-
Abstract
The novel amphiphilic derivatives of Methotrexate–chitosan oligosaccharide MTX–CHO with different molar feeding ratios of MTX were synthesized The degree of MTX substitution ranged from 447 to 135 MTX–CHO copolymer formed micelles with an average size of 1346 ± 1452 to 2366 ± 3001 nm and zeta potential of 20 ± 5 to 168 ± 774 mV The critical micelle concentration was found to range from 125 to 056 mg/l Analysis of micelles with different degree of substitutions DSs revealed that the size of micelles decreased by increasing DS while zeta potential was reduced Release study indicated that drug content had effect on the release rate With increasing amount of loaded drug in the micelle release rate was decreased Drug loaded and unloaded MTX–CHO micelles showed significant cytotoxicity on MDAMB231 Loaded micelle was more effective than unloaded one which indicated that conjugation could reduce efficacy of MTX The viability of MDAMB231 in presence of drug loaded micelles was significantly decreased and cell viability at 1 µg/ml was 4517 ± 9 while the viability of unloaded micelles was 9186 ± 988 These phenomena make MTX–CHO micelles as a good candidate for hydrophobic anticancer drug carrier
Keywords: