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Title of Journal: Cell Tissue Res

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Abbravation: Cell and Tissue Research

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Springer-Verlag

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DOI

10.1007/s40278-015-6863-x

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1432-0878

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ATPevoked increase in intracellular calcium via t

Authors: Haruo Nakano Arata Shimada Kei Imai Toru Takahashi Kazuyoshi Hashizume
Publish Date: 2003/06/28
Volume: 313, Issue: 2, Pages: 227-236
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Abstract

Bovine trophoblasts actively proliferate to elongate blastocysts before implantation The trophoblast at this stage secretes cytokines and starts to differentiate into an endocrine cell binucleate cell for successful pregnancy Intracellular calcium Ca2+i may act as a second messenger in the trophoblast response In this study we investigated Ca2+i signals in a bovine trophoblast cell line BT1 using fura2 fluorescence We found that an application of ATP ≥1 μM induced a transient increase in Ca2+i in BT1 cells The ATPinduced increase was not affected by the removal of extracellular Ca2+ but was suppressed by suramin 100 μM an antagonist of P2 receptors Pretreatment with pertussis toxin 01 or 1 μg/ml partially inhibited the response to ATP The order of potency to increase Ca2+i was ATP=UTPADP ATPinduced Ca2+i responses preferentially occurred in cells at the periphery of the colony The reduced responses at the center of the colony were associated with an increase in cell density and decrease in bromodeoxyuridine incorporation These results indicated that ATP stimulated P2Y receptors coupled to pertussis toxinsensitive and insensitive G proteins leading to an increase in Ca2+i as a result of release of Ca2+ from intracellular stores in BT1 cells The occurrence of ATPinduced Ca2+i signals depended on the cell confluence and reflected the high proliferative activity of the trophoblast cell populationThis work was supported by grants from the Biooriented Technology Research Advancement Institution BRAIN and the Organized Research Combination System in the Science and Technology Agency of Japan HN is a domestic research fellow supported by Japan Society for the Promotion of Science AS is supported by a postdoctoral fellowship from the Japan Science and Technology Corporation

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